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Journal: Scientific Reports
Article Title: Intra-striatal AAV2.retro administration leads to extensive retrograde transport in the rhesus macaque brain: implications for disease modeling and therapeutic development
doi: 10.1038/s41598-020-63559-7
Figure Lengend Snippet: AAV2.retro-mediated retrograde transport is significantly higher compared to the parent serotype, AAV2. ( a ) Low and ( b ) high power photomicrographs of eGFP-stained, rhesus macaque coronal brain sections following injection of AAV2.retro-eGFP (top panel) or AAV2-eGFP (bottom panel) into the caudate and putamen. Rectangles in ( a ) illustrate the brain regions selected for high power photomicrographs displayed in (b ). ( c ) Cell count analysis demonstrating significantly more GFP + cells in the putamen compared to the caudate, irrespective of serotype, as well as significantly more GFP + cells detected in extra-striatal regions in animals injected with AAV2.retro-eGFP compared to AAV2-eGFP. ( d ) Area fraction fractionator analysis showing a significantly higher area fraction of GFP positivity in the putamen in animals injected with AAV2-eGFP compared to AAV2.retro-eGFP. Error bars in both graphs represent standard error of the mean (SEM). Abbreviations: ACC (anterior cingulate cortex), AMY (amygdala), Cd (caudate), DPFC (dorsal prefrontal cortex), DPMC (dorsal premotor cortex), Put (putamen), SSC (somatosensory cortex), THAL (thalamus). *p < 0.05, **p < 0.01, ***p < 0.001. Scale bar 2a = 1 centimeter, Scale bar 2b = 100 microns. Graphs were made in GraphPad Prism for Mac, Version 8.
Article Snippet: To assess these serotype-based differences, we quantified the spread of each vector in the injected regions of the caudate and putamen using the
Techniques: Staining, Injection, Cell Counting